A rapid UPLC-MS/MS method for simultaneous determination of flunitrazepam, 7-aminoflunitrazepam, methadone and EDDP in human, rat and rabbit plasma.

Filed under: Methadone Clinics

Talanta. 2012 Sep 15; 99: 649-59
Kiss B, Bogdan C, Pop A, Loghin F

A simple, high-throughput, sensitive LC-ESI-MS/MS method is presented for the simultaneous determination of methadone (MET), flunitrazepam (FNZ) and their major metabolites, EDDP (2-ethilidene-1,5-dimethyl-3,3-diphenylpyrrolidone) and 7-aminoflunitrazepam (7-AFNZ), respectively, in human, rat and rabbit plasma. The isolation of the selected compounds involved a liquid-liquid extraction with ethyl acetate at a basic pH. Good chromatographic separation was achieved on a HSS T3 column (1.8?m particle size), with a 3min gradient elution using a mixture of acetonitrile with 0.1% formic acid (solvent A) and 5mM ammonium acetate (solvent B) as the mobile phase. The tandem mass spectrometric detection was performed in multiple reaction monitoring (MRM) mode with ionization of the analytes in positive mode. The assay was fully validated according to current acceptance criteria for bioanalytical methods validation. It was proved to be linear in the range of 0.5-250ng/mL, with adequate accuracy and precision over this range. Based on accuracy and CV% values the LOQ and ULOQ values were set at 0.509ng/mL and 2036ng/mL for MET, 0.520ng/mL and 2080ng/mL for EDDP, 0.524ng/mL and 2096ng/mL for FNZ and 0.528ng/mL and 2114ng/mL for 7-AFNZ, respectively. The method was tested for potential matrix effects, without observing significant ion suppression. The investigated compounds stability was examined in plasma at room temperature and after three freeze-thaw cycles and in the final extract when maintained at 4°C in the autosampler. Potential stability issues were observed only for FNZ at room temperature. The method was successfully applied to quantify the selected compounds in human, rat and rabbit plasma samples, after exposure to FNZ or simultaneous exposure to FNZ and MET.
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NICU Nurses’ Lived Experience: Caring for Infants With Neonatal Abstinence Syndrome.

Filed under: Methadone Clinics

Adv Neonatal Care. 2012 Oct; 12(5): 281-5
Maguire D, Webb M, Passmore D, Cline G

: The primary aim of this qualitative methods study was to describe the lived experiences of neonatal intensive care unit (NICU) nurses with ethical and morally challenging issues.: The target population for the study was registered nurses working in the NICU. Interviews were completed with 16 nurses from 1 hospital.: A phenomenological method design was used to describe NICU nurses’ lived experiences with ethical and moral issues encountered in the NICU.: After obtaining signed consent, the principal investigator interviewed all participants, using a semistructured interview guide consisting of open-ended questions.: Ethical and moral distress related to neonatal abstinence syndrome was the predominant outcome.: Caring for infants, coping with families, and discharging infants home were the major concerns voiced by nurses in this study.: Nurses in this study struggled with issues of beneficence and nonmaleficence and were not aware of scientific evidence that guides methadone management of pregnant women.
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Pain clinic owners charged with 66 counts of distributing illegal drugs

Filed under: Methadone Clinics

… with conspiring to illegally distribute and dispense several controlled substances, including oxycodone, methadone, morphine, hydrocodone (Vicoden) and alpzolam (Xanax). The four are also charged with maintaining drug–involved premises (pain clinics).
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